Custom Plasmid
Manufacturing Services
Plasmid Bioscience provides a custom service to manufacture your plasmid DNA by producing the plasmid DNA to your specifications. Together we’ll develop a statement of work agreement to produce the plasmid DNA to yield, purity and quality control.
Custom Plasmid Manufacturing Services
Plasmid Bioscience provides a custom service to manufacture your plasmid DNA by producing the plasmid DNA to your specifications. Together we’ll develop a statement of work agreement to produce the plasmid DNA to yield, purity and quality control.
Purity Grades
| Plasmid DNA Manufacturing Services | Scale | Application | Production |
| Research Grade | 100ug-2.5mg | In vitro R&D or preclinical studies | R&D for cloning, vector construction, optimizations, protein expression and transfection |
| Development Grade | 100ug-1g | In vitro Research and Development studies | Low Endo Toxins for sensitive cells |
| Pre-Clinical Grade | >1g | Toxicology, vector production optimization for clinical trials, and as template for mRNA production | Dedicated Segregated Systems and Quality Control |
Quality Control
| QC Items | Specification | Method |
| A260/280 | 1.8-2.0 | UV Absorption |
| Appearance | Clear | Visual Inspection |
| Concentration | Per SOW | UV Absorption |
| Supercoil content | >80% | Agarose Gel |
| Residue RNA | Not Visible | Agarose Gel |
| Genomic DNA | Not Visible | Agarose Gel |
| Restriction Digest | Matches Reference | Agarose Gel |
| Endotoxin Assay | 0.1 EU/ug plasmid DNA | LAL Assay |
| BioBurden Assay | No Growth | Agar plate for two days |
| ITR Integrity | ITR cuts and is visible on Agarose Gel | Enzymatic Digestion and Agarose Gel |
Purity Grades
Quality Control
